Publication: Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D
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2017-10-01
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18767753
18735061
18735061
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2-s2.0-85029441927
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item.page.oaire.edition
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Mahidol University
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Stem Cell Research. Vol.24, (2017), 21-24
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Warunya Chakritbudsabong, Ladawan Sariya, Sirikron Pamonsupornvichit, Rassmeepen Pronarkngver, Somjit Chaiwattanarungruengpaisan, Joao N. Ferreira, Piyathip Setthawong, Praopilas Phakdeedindan, Mongkol Techakumphu, Theerawat Tharasanit, Sasitorn Rungarunlert (2017). Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41752.
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Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D
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Abstract
© 2017 The Authors Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo. Upon cardiac differentiation, VSMUi001-D displayed spontaneous beating and expressed cardiomyocyte markers, like cardiac Troponin T.