Antiproliferative effect and the isolated compounds of pouzolzia indica

Abstract

Previous report showed the high potent antiproliferative effect of the methanolic part extracted from the aerial parts of Pouzolzia indica on NB4 and HT93A acute leukemic cell lines with the IC50values of 28.5 and 49.8 g/mL, respectively. The bioassay-guided fractionation of the methanolic part gave 5 fractions, that is, FFI-FFV. FFII, FFIII, and FFIV inhibited the above leukemic cell lines with the IC50values of 15.1 (FFII), 14.4 (FFIII), 32.1 (FFIV), and 31.0 (FFII), 9.7 (FFIII), 10.5 (FFIV) g/mL, respectively. The compounds in these fractions were isolated using chromatographic technique. FFII contained friedelin 1, 28-hydroxy-3-friedelanone 2, and 7-methoxy-coumarin 3. FFIII contained 6, 7-dimethoxy-coumarin 4, scopoletin 5, methyl caffeate 6. FFIV contained sitosteryl glucoside 7 and a supposed glycosphingolipid 8. The chemical structures were elucidated by spectroscopic methods. © 2013 Chanyapat Sangsuwon et al.