Publication: Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms
Issued Date
2008-05-01
Resource Type
ISSN
00992240
Other identifier(s)
2-s2.0-44249127260
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Applied and Environmental Microbiology. Vol.74, No.10 (2008), 3302-3305
Suggested Citation
Mark R. Liles, Lynn L. Williamson, Jitsupang Rodbumrer, Vigdis Torsvik, Robert M. Goodman, Jo Handelsman Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms. Applied and Environmental Microbiology. Vol.74, No.10 (2008), 3302-3305. doi:10.1128/AEM.02630-07 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18935
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Title
Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms
Abstract
We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries. Copyright © 2008, American Society for Microbiology. All Rights Reserved.