Publication: Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms
| dc.contributor.author | Mark R. Liles | en_US |
| dc.contributor.author | Lynn L. Williamson | en_US |
| dc.contributor.author | Jitsupang Rodbumrer | en_US |
| dc.contributor.author | Vigdis Torsvik | en_US |
| dc.contributor.author | Robert M. Goodman | en_US |
| dc.contributor.author | Jo Handelsman | en_US |
| dc.contributor.other | Auburn University | en_US |
| dc.contributor.other | University of Wisconsin Madison | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | Universitetet i Bergen | en_US |
| dc.contributor.other | School of Environmental and Biological Sciences | en_US |
| dc.contributor.other | Rutgers, The State University of New Jersey | en_US |
| dc.date.accessioned | 2018-07-12T02:18:58Z | |
| dc.date.available | 2018-07-12T02:18:58Z | |
| dc.date.issued | 2008-05-01 | en_US |
| dc.description.abstract | We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries. Copyright © 2008, American Society for Microbiology. All Rights Reserved. | en_US |
| dc.identifier.citation | Applied and Environmental Microbiology. Vol.74, No.10 (2008), 3302-3305 | en_US |
| dc.identifier.doi | 10.1128/AEM.02630-07 | en_US |
| dc.identifier.issn | 00992240 | en_US |
| dc.identifier.other | 2-s2.0-44249127260 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/18935 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=44249127260&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.subject | Environmental Science | en_US |
| dc.subject | Immunology and Microbiology | en_US |
| dc.title | Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=44249127260&origin=inward | en_US |
