Publication: Evaluation of a genus-specific rGroEL<inf>1-524</inf> IgM-ELISA and commercial ELISA kits during the course of leptospirosis in Thailand
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Issued Date
2021-12-01
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video/youtube
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20452322
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2-s2.0-85116388324
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Mahidol University
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SCOPUS
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Scientific Reports. Vol.11, No.1 (2021)
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Santi Maneewatchararangsri, Galayanee Doungchawee, Thareerat Kalambaheti, Viravarn Luvira, Ngamphol Soonthornworasiri, Pisut Vattanatham, Urai Chaisri, Poom Adisakwattana Evaluation of a genus-specific rGroEL<inf>1-524</inf> IgM-ELISA and commercial ELISA kits during the course of leptospirosis in Thailand
. Scientific Reports. Vol.11, No.1 (2021). doi:10.1038/s41598-021-99377-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/79210
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Title
Evaluation of a genus-specific rGroEL<inf>1-524</inf> IgM-ELISA and commercial ELISA kits during the course of leptospirosis in Thailand
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Abstract
In the present study, we developed a genus-specific rGroEL1-524 IgM-ELISA assay for use in screening diagnosis of suspected leptospirosis among acute undifferentiated febrile illness patients during acute fever. The diagnostic accuracies of the rGroEL1–524 IgM-ELISA, commercial Panbio IgM-ELISA, and Virion-Serion Classic IgG-ELISA were evaluated using 133 Thai leptospirosis sera and 210 controls. Sensitivities were 91.7%, 59.6%, and 17.7% for acute infection, and the specificities were 92.6%, 90.2%, and 88.3% for the non-leptospirosis control, respectively. The rGroEL1-524 IgM-ELISA had high sensitivity, at 92.3% and 91.7%, among culture-positive and MAT-negative cases at 1–3 days post-onset of symptoms (DPO1–3), respectively. Impaired specificity on scrub typhus was found, possibly from antibody cross-reaction to ortholog GroEL. Commercial Panbio IgM-ELISA had sensitivities at DPO1–3 of 30.8% and 41.7% for culture-positive and MAT-negative cases whereas Virion-Serion IgG-ELISA showed sensitivities of 5.9% and 13.3%, respectively. The rGroEL1-524 IgM-ELISA could be useful as a screening test for early diagnosis. The performance of the commercial ELISA suggests the applicability of IgM-ELISA for diagnosis, while IgG-ELISA is useful for seroprevalence surveys. However, confirmation by reference tests is recommended.