Publication: A simplified positive-sense-RNA virus construction approach that enhances analysis throughput
Issued Date
2013-12-01
Resource Type
ISSN
10985514
0022538X
0022538X
Other identifier(s)
2-s2.0-84887207401
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virology. Vol.87, No.23 (2013), 12667-12674
Suggested Citation
Bunpote Siridechadilok, Methee Gomutsukhavadee, Thunyarat Sawaengpol, Sutha Sangiambut, Chunya Puttikhunt, Kwanrutai Chin-inmanu, Prapat Suriyaphol, Prida Malasit, Gavin Screaton, Juthathip Mongkolsapaya A simplified positive-sense-RNA virus construction approach that enhances analysis throughput. Journal of Virology. Vol.87, No.23 (2013), 12667-12674. doi:10.1128/JVI.02261-13 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31826
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Title
A simplified positive-sense-RNA virus construction approach that enhances analysis throughput
Abstract
Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses. © 2013, American Society for Microbiology.