Publication: A simplified positive-sense-RNA virus construction approach that enhances analysis throughput
| dc.contributor.author | Bunpote Siridechadilok | en_US |
| dc.contributor.author | Methee Gomutsukhavadee | en_US |
| dc.contributor.author | Thunyarat Sawaengpol | en_US |
| dc.contributor.author | Sutha Sangiambut | en_US |
| dc.contributor.author | Chunya Puttikhunt | en_US |
| dc.contributor.author | Kwanrutai Chin-inmanu | en_US |
| dc.contributor.author | Prapat Suriyaphol | en_US |
| dc.contributor.author | Prida Malasit | en_US |
| dc.contributor.author | Gavin Screaton | en_US |
| dc.contributor.author | Juthathip Mongkolsapaya | en_US |
| dc.contributor.other | Thailand National Center for Genetic Engineering and Biotechnology | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | Imperial College London | en_US |
| dc.date.accessioned | 2018-10-19T04:59:40Z | |
| dc.date.available | 2018-10-19T04:59:40Z | |
| dc.date.issued | 2013-12-01 | en_US |
| dc.description.abstract | Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses. © 2013, American Society for Microbiology. | en_US |
| dc.identifier.citation | Journal of Virology. Vol.87, No.23 (2013), 12667-12674 | en_US |
| dc.identifier.doi | 10.1128/JVI.02261-13 | en_US |
| dc.identifier.issn | 10985514 | en_US |
| dc.identifier.issn | 0022538X | en_US |
| dc.identifier.other | 2-s2.0-84887207401 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/123456789/31826 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84887207401&origin=inward | en_US |
| dc.subject | Immunology and Microbiology | en_US |
| dc.title | A simplified positive-sense-RNA virus construction approach that enhances analysis throughput | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84887207401&origin=inward |