Publication: Identification of mokB involved in monacolin K biosynthesis in Monascus pilosus
Issued Date
2009-11-01
Resource Type
ISSN
15736776
01415492
01415492
Other identifier(s)
2-s2.0-73949088012
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biotechnology Letters. Vol.31, No.12 (2009), 1911-1916
Suggested Citation
Kanae Sakai, Hiroshi Kinoshita, Takuya Nihira Identification of mokB involved in monacolin K biosynthesis in Monascus pilosus. Biotechnology Letters. Vol.31, No.12 (2009), 1911-1916. doi:10.1007/s10529-009-0093-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27122
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Identification of mokB involved in monacolin K biosynthesis in Monascus pilosus
Author(s)
Other Contributor(s)
Abstract
Monacolin K (MK), which is widely used as an antihypercholesterolemia medicine, is produced as a fungal secondary metabolite through the polyketide pathway. The MK biosynthetic gene cluster proposed for Monascus pilosus BCRC38072 was also identified in M. pilosus NBRC4480. The mokB gene, located at the end of the putative gene cluster and possibly encoding polyketide synthase, was disrupted. The mokB disruptant did not produce MK, but accumulated an intermediate that was confirmed to be monacolin J, indicating that mokB encodes the polyketide synthase responsible for the biosynthesis of side-chain diketide moiety. © Springer Science+Business Media B.V. 2009.