Publication: Controlled-release biocatalysis for the synthesis of D-phenylglycine
Issued Date
2004-05-01
Resource Type
ISSN
10242422
Other identifier(s)
2-s2.0-4844219951
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Mahidol University
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SCOPUS
Bibliographic Citation
Biocatalysis and Biotransformation. Vol.22, No.3 (2004), 195-201
Suggested Citation
T. Rojanarata, D. Isarangkul, S. Wiyakrutta, V. Meevootisom, J. M. Woodley Controlled-release biocatalysis for the synthesis of D-phenylglycine. Biocatalysis and Biotransformation. Vol.22, No.3 (2004), 195-201. doi:10.1080/10242420410001727355 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21194
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Title
Controlled-release biocatalysis for the synthesis of D-phenylglycine
Abstract
An isolated and immobilised aminotransferase cloned from Pseudomonas stutzeri ST-201 into Escherichia coli was used to synthesise D-phenylglycine. The reaction was characterised by an unfavourable equilibrium constant and substrate inhibition. The use of a controlled-release system via the use of Amberlite (IRA 400)-adsorbed benzoylformate proved a useful technique to circumvent these issues. This resulted in a four-fold improvement in product concentration achievable to yield a final D-phenylglycine concentration of 10.25 mg/ml. © 2004 Taylor & Francis Ltd.