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Controlled-release biocatalysis for the synthesis of D-phenylglycine

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dc.contributor.authorT. Rojanarataen_US
dc.contributor.authorD. Isarangkulen_US
dc.contributor.authorS. Wiyakruttaen_US
dc.contributor.authorV. Meevootisomen_US
dc.contributor.authorJ. M. Woodleyen_US
dc.contributor.otherUCLen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThe Institute of Science and Technology for Research and Development, Mahidol Universityen_US
dc.date.accessioned2018-07-24T03:37:43Z
dc.date.available2018-07-24T03:37:43Z
dc.date.issued2004-05-01en_US
dc.description.abstractAn isolated and immobilised aminotransferase cloned from Pseudomonas stutzeri ST-201 into Escherichia coli was used to synthesise D-phenylglycine. The reaction was characterised by an unfavourable equilibrium constant and substrate inhibition. The use of a controlled-release system via the use of Amberlite (IRA 400)-adsorbed benzoylformate proved a useful technique to circumvent these issues. This resulted in a four-fold improvement in product concentration achievable to yield a final D-phenylglycine concentration of 10.25 mg/ml. © 2004 Taylor & Francis Ltd.en_US
dc.identifier.citationBiocatalysis and Biotransformation. Vol.22, No.3 (2004), 195-201en_US
dc.identifier.doi10.1080/10242420410001727355en_US
dc.identifier.issn10242422en_US
dc.identifier.other2-s2.0-4844219951en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/21194
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=4844219951&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemical Engineeringen_US
dc.titleControlled-release biocatalysis for the synthesis of D-phenylglycineen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=4844219951&origin=inwarden_US

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