Publication: Thrombomodulin exerts cytoprotective effect on low-dose UVB-irradiated HaCaT cells
Issued Date
2008-12-12
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-55549140799
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.377, No.2 (2008), 642-647
Suggested Citation
Masahiro Iwata, Ko ichi Kawahara, Hisashi Kawabata, Takashi Ito, Kentaro Mera, Kamal Krishna Biswas, Salunya Tancharoen, Yuko Higashi, Kiyoshi Kikuchi, Teruto Hashiguchi, Takuro Kanekura, Ikuro Maruyama Thrombomodulin exerts cytoprotective effect on low-dose UVB-irradiated HaCaT cells. Biochemical and Biophysical Research Communications. Vol.377, No.2 (2008), 642-647. doi:10.1016/j.bbrc.2008.10.049 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18799
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Title
Thrombomodulin exerts cytoprotective effect on low-dose UVB-irradiated HaCaT cells
Abstract
Thrombomodulin (TM) is an endothelial cell surface anticoagulant glycoprotein that performs antimetastatic, angiogenic, adhesive, and anti-inflammatory functions in various tissues. It is also expressed in epidermal keratinocytes. We found that a physiological dose (10 mJ/cm2) of mid-wavelength ultraviolet irradiation (UVB) significantly induced TM expression via the p38mitogen-activated protein kinase (MAPK)/cyclic AMP response element (CRE) signaling pathway in the epidermal keratinocyte cell line HaCaT; this shows that TM regulates the survival of HaCaT cells. SB203580, a p38MAPK inhibitor, significantly decreased TM expression and the viability of cells exposed to UVB. Furthermore, overexpression of TM markedly increased cell viability, and it was abrogated by TM small interfering RNA (siRNA), suggesting that TM may play an important role in exerting cytoprotective effect on epidermal keratinocytes against low-dose UVB. © 2008 Elsevier Inc. All rights reserved.