Publication:
Generation of B2M bi-allelic knockout human induced pluripotent stem cells (MUSIi001-A-1) using a CRISPR/Cas9 system

2

Issued Date

2021-10-01

Resource Type

Article

ISSN

18767753
18735061

DOI

10.1016/j.scr.2021.102551

Other identifier(s)

2-s2.0-85116659144

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Stem Cell Research. Vol.56, (2021)

Suggested Citation

Nontaphat Thongsin, Methichit Wattanapanitch Generation of B2M bi-allelic knockout human induced pluripotent stem cells (MUSIi001-A-1) using a CRISPR/Cas9 system. Stem Cell Research. Vol.56, (2021). doi:10.1016/j.scr.2021.102551 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76007

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Title

Generation of B2M bi-allelic knockout human induced pluripotent stem cells (MUSIi001-A-1) using a CRISPR/Cas9 system

Author(s)

Nontaphat Thongsin
 Methichit Wattanapanitch

Other Contributor(s)

Siriraj Hospital

Abstract

Allogeneic cell-based therapy is emerging as a promising approach in regenerative medicine. However, rejection of allograft due to mismatch of human leukocyte antigens (HLAs) remains a major concern after transplantation. Here, we generated a homozygous B2M knockout induced pluripotent stem cell (iPSC) line, lacking the expression of HLA class I (HLA-I) molecules, using a CRISPR/Cas9 system. The established iPSC line, MUSIi001-A-1, can serve as an in vitro model for studying immunological responses against allogeneic grafts and provides a prototype for “off-the-shelf” allogeneic cell products for future cell-based therapy.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/76007

Collections

Scopus 2021