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Generation of B2M bi-allelic knockout human induced pluripotent stem cells (MUSIi001-A-1) using a CRISPR/Cas9 system

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dc.contributor.authorNontaphat Thongsinen_US
dc.contributor.authorMethichit Wattanapanitchen_US
dc.contributor.otherSiriraj Hospitalen_US
dc.date.accessioned2022-08-04T08:05:11Z
dc.date.available2022-08-04T08:05:11Z
dc.date.issued2021-10-01en_US
dc.description.abstractAllogeneic cell-based therapy is emerging as a promising approach in regenerative medicine. However, rejection of allograft due to mismatch of human leukocyte antigens (HLAs) remains a major concern after transplantation. Here, we generated a homozygous B2M knockout induced pluripotent stem cell (iPSC) line, lacking the expression of HLA class I (HLA-I) molecules, using a CRISPR/Cas9 system. The established iPSC line, MUSIi001-A-1, can serve as an in vitro model for studying immunological responses against allogeneic grafts and provides a prototype for “off-the-shelf” allogeneic cell products for future cell-based therapy.en_US
dc.identifier.citationStem Cell Research. Vol.56, (2021)en_US
dc.identifier.doi10.1016/j.scr.2021.102551en_US
dc.identifier.issn18767753en_US
dc.identifier.issn18735061en_US
dc.identifier.other2-s2.0-85116659144en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/76007
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85116659144&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleGeneration of B2M bi-allelic knockout human induced pluripotent stem cells (MUSIi001-A-1) using a CRISPR/Cas9 systemen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85116659144&origin=inwarden_US

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