Publication:
The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum

Issued Date

2011-03-01

Resource Type

Article

ISSN

10986596
00664804

DOI

10.1128/AAC.01103-10

Other identifier(s)

2-s2.0-79952347602

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Antimicrobial Agents and Chemotherapy. Vol.55, No.3 (2011), 1300-1304

Suggested Citation

S. Kaewpongsri, K. Sriprawat, R. Suwanarusk, D. E. Kyle, U. Lek-Uthai, M. Leimanis, K. M. Lwin, A. P. Phyo, J. Zwang, B. Russell, F. Nosten, L. Renia The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum. Antimicrobial Agents and Chemotherapy. Vol.55, No.3 (2011), 1300-1304. doi:10.1128/AAC.01103-10 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12627

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Title

The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum

Author(s)

S. Kaewpongsri
 K. Sriprawat
 R. Suwanarusk
 D. E. Kyle
 U. Lek-Uthai
 M. Leimanis
 K. M. Lwin
 A. P. Phyo
 J. Zwang
 B. Russell
 F. Nosten
 L. Renia

Other Contributor(s)

Shoklo Malaria Research Unit
 Agency for Science, Technology and Research, Singapore
 University of South Florida Tampa
 Mahidol University
 John Radcliffe Hospital

Abstract

Plasmodium species ex vivo sensitivity assay protocols differ in the requirement for leukocyte removal before culturing. This study shows that the presence of leukocytes significantly increases the 50% inhibitory concentration (IC 50 ) of P. vivax and P. falciparum to artesunate and chloroquine relative to results with the paired leukocyte-free treatment. Although leukocyte removal is not an essential requirement for the conduct of ex vivo assays, its use has important implications for the interpretation of temporal and spatial antimalarial sensitivity data. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

Keyword(s)

Medicine
 Pharmacology, Toxicology and Pharmaceutics

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/12627

Collections

Scopus 2011-2015