Publication: Probiotic bacteria (Lactobacillus plantarum) expressing specific double-stranded RNA and its potential for controlling shrimp viral and bacterial diseases
10
Issued Date
2017-10-01
Resource Type
ISSN
1573143X
09676120
09676120
Other identifier(s)
2-s2.0-85017155222
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Mahidol University
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SCOPUS
Bibliographic Citation
Aquaculture International. Vol.25, No.5 (2017), 1679-1692
Suggested Citation
Thitiporn Thammasorn, Sarocha Jitrakorn, Patai Charoonnart, Suwitchaya Sirimanakul, Triwit Rattanarojpong, Soraya Chaturongakul, Vanvimon Saksmerprome Probiotic bacteria (Lactobacillus plantarum) expressing specific double-stranded RNA and its potential for controlling shrimp viral and bacterial diseases. Aquaculture International. Vol.25, No.5 (2017), 1679-1692. doi:10.1007/s10499-017-0144-z Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41332
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Title
Probiotic bacteria (Lactobacillus plantarum) expressing specific double-stranded RNA and its potential for controlling shrimp viral and bacterial diseases
Abstract
© 2017, Springer International Publishing Switzerland. We engineered probiotic bacteria, Lactobacillus plantarum, to produce specific double-stranded (ds)RNA for viral inhibition in penaeid shrimp. When supplemented in feed, the developed strain should offer specific viral disease resistance and still remain other beneficial effects on shrimp health against other bacterial pathogens. The pWH1520 expression vector was constructed to encode hairpin RNA targeting shrimp yellow head virus (YHV) in L. plantarum. The quantity of YHV-specific dsRNA (dsYHV) was approximately 1.66 ± 0.25 ng from 7 × 1011 CFU of L. plantarum. Feeding shrimp with the probiotic expressing dsYHV (at 1011 CFU g−1feed) for 5 days prior to YHV challenge provided shrimp partial protection against YHV. In vitro agar well diffusion analysis revealed that both wild-type and transformed L. plantarum inhibited growth of Vibrio parahaemolyticus, the shrimp pathogen responsible for acute hepatopancreatic necrosis disease (AHPND) and early mortality syndrome (EMS). When tested in shrimp by immersion method, both L. plantarum strains (at 107 CFUs ml−1seawater) also provided shrimp protection against V. parahaemolyticus. In conclusion, this study suggested the potential of the dsRNA-expressed L. plantarum for promoting shrimp health against the pathogenic virus. In addition, engineering L. plantarum for dsRNA production did not alter the probiotic’s intrinsic antibacterial property, and therefore, the developed strain can be used to protect shrimp against the important bacterial pathogen, V. parahaemolyticus causing AHPND/EMS.