Asian Pacific Journal of Allergy and Immunology. Vol.19, No.1 (2001), 43-48
Suggested Citation
K. Sangsiriwut, P. Auewarakul, S. Suwanagool, C. Wasi Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay. Asian Pacific Journal of Allergy and Immunology. Vol.19, No.1 (2001), 43-48. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26558
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Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay
A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count rage of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 106 CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts.
