Publication: Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay
| dc.contributor.author | K. Sangsiriwut | en_US |
| dc.contributor.author | P. Auewarakul | en_US |
| dc.contributor.author | S. Suwanagool | en_US |
| dc.contributor.author | C. Wasi | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.date.accessioned | 2018-09-07T09:41:23Z | |
| dc.date.available | 2018-09-07T09:41:23Z | |
| dc.date.issued | 2001-08-14 | en_US |
| dc.description.abstract | A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count rage of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 106 CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts. | en_US |
| dc.identifier.citation | Asian Pacific Journal of Allergy and Immunology. Vol.19, No.1 (2001), 43-48 | en_US |
| dc.identifier.issn | 0125877X | en_US |
| dc.identifier.other | 2-s2.0-0034916313 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/26558 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034916313&origin=inward | en_US |
| dc.subject | Immunology and Microbiology | en_US |
| dc.subject | Medicine | en_US |
| dc.title | Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034916313&origin=inward | en_US |