Publication: Physicochemical properties of amphotericin B liposomes prepared by reverse-phase evaporation method
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Issued Date
2003-02-24
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ISSN
03639045
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2-s2.0-0037276589
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Mahidol University
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SCOPUS
Bibliographic Citation
Drug Development and Industrial Pharmacy. Vol.29, No.1 (2003), 31-37
Suggested Citation
Pleumchitt Rojanapanthu, Narong Sarisuta, Korakot Chaturon, Krisana Kraisintu Physicochemical properties of amphotericin B liposomes prepared by reverse-phase evaporation method. Drug Development and Industrial Pharmacy. Vol.29, No.1 (2003), 31-37. doi:10.1081/DDC-120016681 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20761
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Title
Physicochemical properties of amphotericin B liposomes prepared by reverse-phase evaporation method
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Abstract
The physicochemical properties of phosphatidylcholine-cholesterol liposomes containing amphotericin B and prepared by reverse-phase evaporation method were studied. Uniformly dispersed liposomal suspensions were obtained by employing 3:1 ratio (by volume) of diethyl ether to normal saline, 5 min sonication time at 7°C, and evaporation of diethyl ether at 25°C. Microscopic examination showed that the prepared liposomes were spheroids with unilamellar, oligolamellar, or multilamellar structure. The liposomes containing amphotericin B 2.0 mol% of total lipid led to the highest percentage of drug entrapment. Liposomes with maximum entrapment efficiency were obtained from using 250 μmol of total lipid. The liposomal amphotericin B possessing the highest drug entrapment efficiency (approximately 95%) with particle size range of 1307-1451 nm was the one composed of 1:1 molar ratio of phosphatidylcholine to cholesterol.