Publication: Native troponin-T of the American cock-roach (CR), Periplaneta americana, binds to IgE in sera of CR allergic Thais
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Issued Date
2007-12-01
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ISSN
0125877X
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2-s2.0-40749135061
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.25, No.4 (2007), 189-197
Suggested Citation
Onruedee Khantisitthiporn, Nitat Sookrung, Anchalee Tungtrongchitr, Pongsri Tongtawe, Chaweewan Bunnag, Potjanee Srimanote, Pramuan Tapchaisri, Wanpen Chaicumpa Native troponin-T of the American cock-roach (CR), Periplaneta americana, binds to IgE in sera of CR allergic Thais. Asian Pacific Journal of Allergy and Immunology. Vol.25, No.4 (2007), 189-197. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24482
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Title
Native troponin-T of the American cock-roach (CR), Periplaneta americana, binds to IgE in sera of CR allergic Thais
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Abstract
The American cockroach, Periplaneta americana, is the predominant cockroach (CR) species in Thailand and a major source of indoor allergens second only to the house dust mite. The incidence of CR allergy among allergic Thai patients is increasing but basic information on the allergenic components is scarce. In this study a recombinant troponin-T was produced by using cDNA prepared from RNA of the P. americana as a template and PCR primers designed from the P. americana troponin-T sequence deposited in the GenBank database. The recominant protein (Mr ∼50) did not bind to IgE in the sera of 18 skin prick test positive CR allergic patients. Rabbit polyclonal antiserum (PAb) against the recombinant troponin-T was produced and used in preparing an affinity column for the purification of native troponin-T from the crude P. americana extract (Mr ∼47). IgE-immunobloting revealed that the native protein bound to IgE in 3 of the 18 (16.7%) patients. Our results imply that native P. americana troponin-T, but not its recombinant counterpart, is a minor allergen among the CR allergic Thais.