Publication:
Native troponin-T of the American cock-roach (CR), Periplaneta americana, binds to IgE in sera of CR allergic Thais

dc.contributor.authorOnruedee Khantisitthipornen_US
dc.contributor.authorNitat Sookrungen_US
dc.contributor.authorAnchalee Tungtrongchitren_US
dc.contributor.authorPongsri Tongtaween_US
dc.contributor.authorChaweewan Bunnagen_US
dc.contributor.authorPotjanee Srimanoteen_US
dc.contributor.authorPramuan Tapchaisrien_US
dc.contributor.authorWanpen Chaicumpaen_US
dc.contributor.otherThammasat Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-08-24T01:51:02Z
dc.date.available2018-08-24T01:51:02Z
dc.date.issued2007-12-01en_US
dc.description.abstractThe American cockroach, Periplaneta americana, is the predominant cockroach (CR) species in Thailand and a major source of indoor allergens second only to the house dust mite. The incidence of CR allergy among allergic Thai patients is increasing but basic information on the allergenic components is scarce. In this study a recombinant troponin-T was produced by using cDNA prepared from RNA of the P. americana as a template and PCR primers designed from the P. americana troponin-T sequence deposited in the GenBank database. The recominant protein (Mr ∼50) did not bind to IgE in the sera of 18 skin prick test positive CR allergic patients. Rabbit polyclonal antiserum (PAb) against the recombinant troponin-T was produced and used in preparing an affinity column for the purification of native troponin-T from the crude P. americana extract (Mr ∼47). IgE-immunobloting revealed that the native protein bound to IgE in 3 of the 18 (16.7%) patients. Our results imply that native P. americana troponin-T, but not its recombinant counterpart, is a minor allergen among the CR allergic Thais.en_US
dc.identifier.citationAsian Pacific Journal of Allergy and Immunology. Vol.25, No.4 (2007), 189-197en_US
dc.identifier.issn0125877Xen_US
dc.identifier.other2-s2.0-40749135061en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/24482
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=40749135061&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleNative troponin-T of the American cock-roach (CR), Periplaneta americana, binds to IgE in sera of CR allergic Thaisen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=40749135061&origin=inwarden_US

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