Publication: Misclassification of drug failure in plasmodium falciparum clinical trials in southeast asia
Issued Date
2009-08-15
Resource Type
ISSN
00221899
DOI
Other identifier(s)
2-s2.0-69149107186
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Infectious Diseases. Vol.200, No.4 (2009), 624-628
Suggested Citation
Jonathan J. Juliano, Frederic Ariey, Rithy Sem, Noppadon Tangpukdee, Srivicha Krudsood, Carol Olson, Sornchai Looareesuwan, William O. Rogers, Chansuda Wongsrichanalai, Steven R. Meshnick Misclassification of drug failure in plasmodium falciparum clinical trials in southeast asia. Journal of Infectious Diseases. Vol.200, No.4 (2009), 624-628. doi:10.1086/600892 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27981
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Title
Misclassification of drug failure in plasmodium falciparum clinical trials in southeast asia
Abstract
Most trials of antimalarials occur in areas in which reinfections are possible. For Plasmodium falciparum, reinfections are distinguished from recrudescences by polymerase chain reaction analysis of 3 polymorphic genes. However, the validity of this approach has never been rigorously tested. We tested for misclassification in 6 patients from clinical trials in Thailand and Cambodia who were classified as being reinfected by the standard polymerase chain reaction protocol. Using heteroduplex tracking assays and direct DNA sequencing, we found that 5 (83%) of 6 patients were misclassified. Misclassification in this manner overestimates the efficacy of antimalarials and delays the recognition of decreasing therapeutic efficacy, thus delaying potential changes in policy. © 2009 by the Infectious Diseases Society of America. All rights reserved.