Publication: Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes
Issued Date
2018-07-01
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ISSN
20928467
1738642X
1738642X
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2-s2.0-85060332500
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Cellular Toxicology. Vol.14, No.3 (2018), 273-282
Suggested Citation
Preeyaporn Koedrith, Yeo Jin Kim, Younghun Kim, Joo Hyon Kang, Young Rok Seo Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes. Molecular and Cellular Toxicology. Vol.14, No.3 (2018), 273-282. doi:10.1007/s13273-018-0030-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45878
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Title
Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes
Abstract
© 2018, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V. Backgrounds: Trends in the use of an anticoagulant as a dispersing stabilizer are addressed. An effective approach to preparing stable nanosized titanium dioxide (nTiO 2 ) for accurate and systematic assessment of nano- toxicity has not been established. Methods: Among the dispersants tested here, it was found that sodium polyacrylate (PAA) was the most effective dispersant for nTiO 2 in culture media. Our study was the first to demonstrate that a stable PAA-dispersed nTiO 2 (nTiO 2 /PAA) suspension showed more toxic than nTiO 2 without PAA in human HaCaT keratinocytes. Results: Initially, MTT results showed that the stable nTiO 2 /PAA dispersion exhibited significantly greater cytotoxicity than nTiO 2 without PAA. In addition, the stable nTiO 2 /PAA dispersion induced markedly more oxidative stress than nTiO 2 without PAA. Importantly, the stable nTiO 2 /PAA dispersion caused DNA breakage to a greater extent than nTiO 2 without PAA. Conclusion: Our findings indicated that the anti-coagulant PAA is suitable for preparing homologous dispersed nTiO 2 under realistic physiological culture test conditions.