Publication: Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells
Issued Date
2004-05-01
Resource Type
ISSN
08824010
Other identifier(s)
2-s2.0-1642362209
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microbial Pathogenesis. Vol.36, No.5 (2004), 287-292
Suggested Citation
Wannapa Kespichayawattana, Pakamas Intachote, Pongsak Utaisincharoen, Stitaya Sirisinha Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells. Microbial Pathogenesis. Vol.36, No.5 (2004), 287-292. doi:10.1016/j.micpath.2004.01.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21387
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells
Other Contributor(s)
Abstract
Burkholderia pseudomallei, a causative agent of melioidosis, is a facultative intracellular gram-negative bacillus that is closely related to its avirulent counterpart, Burkholderia thailandensis. However, pathogenic mechanisms and virulence factors of B. pseudomallei remain elusive. In the present study, we compared the invasiveness, adherence, and replication of B. pseudomallei and B. thailandensis in human respiratory epithelial cells A549. Invasion was determined after 4 h of coculturing using antibiotic protection assay. Adherence was demonstrated by coculturing the cells with fluorescein-labeled bacteria for 1 h and the number of positive cells was analyzed by flow cytometry. The results obtained with this in vitro study demonstrated that compared with its avirulent counterpart, B. pseudomallei is significantly more efficient (P<0.01) in invasion, adherence and inducing cellular damage, as represented by plaque formation. © 2004 Elsevier Ltd. All rights reserved.