Publication:
Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells

dc.contributor.authorWannapa Kespichayawattanaen_US
dc.contributor.authorPakamas Intachoteen_US
dc.contributor.authorPongsak Utaisincharoenen_US
dc.contributor.authorStitaya Sirisinhaen_US
dc.contributor.otherChulabhorn Research Instituteen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-07-24T03:43:45Z
dc.date.available2018-07-24T03:43:45Z
dc.date.issued2004-05-01en_US
dc.description.abstractBurkholderia pseudomallei, a causative agent of melioidosis, is a facultative intracellular gram-negative bacillus that is closely related to its avirulent counterpart, Burkholderia thailandensis. However, pathogenic mechanisms and virulence factors of B. pseudomallei remain elusive. In the present study, we compared the invasiveness, adherence, and replication of B. pseudomallei and B. thailandensis in human respiratory epithelial cells A549. Invasion was determined after 4 h of coculturing using antibiotic protection assay. Adherence was demonstrated by coculturing the cells with fluorescein-labeled bacteria for 1 h and the number of positive cells was analyzed by flow cytometry. The results obtained with this in vitro study demonstrated that compared with its avirulent counterpart, B. pseudomallei is significantly more efficient (P<0.01) in invasion, adherence and inducing cellular damage, as represented by plaque formation. © 2004 Elsevier Ltd. All rights reserved.en_US
dc.identifier.citationMicrobial Pathogenesis. Vol.36, No.5 (2004), 287-292en_US
dc.identifier.doi10.1016/j.micpath.2004.01.001en_US
dc.identifier.issn08824010en_US
dc.identifier.other2-s2.0-1642362209en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/21387
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=1642362209&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleVirulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cellsen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=1642362209&origin=inwarden_US

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