Publication:
The reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycle

Issued Date

2013-12-06

Resource Type

Article

ISSN

1083351X
00219258

DOI

10.1074/jbc.M113.515205

Other identifier(s)

2-s2.0-84894312047

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Biological Chemistry. Vol.288, No.49 (2013), 35222-35236

Suggested Citation

Jeerus Sucharitakul, Chanakan Tongsook, Danaya Pakotiprapha, Willem J.H. Van Berkel, Pimchai Chaiyen The reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycle. Journal of Biological Chemistry. Vol.288, No.49 (2013), 35222-35236. doi:10.1074/jbc.M113.515205 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31142

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Title

The reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycle

Author(s)

Jeerus Sucharitakul
 Chanakan Tongsook
 Danaya Pakotiprapha
 Willem J.H. Van Berkel
 Pimchai Chaiyen

Other Contributor(s)

Chulalongkorn University
 Mahidol University
 Wageningen University and Research Centre

Abstract

Background: 3-Hydroxybenzoate 6-hydroxylase (3HB6H) is a flavoprotein hydroxylase catalyzing the para-hydroxylation of 3-hydroxybenzoate. Results: The oxidative half-reaction was studied using transient kinetics. The enzyme reaction mechanism was elucidated. Conclusion: The product release and the hydroxylation limit the turnovers and the enzyme shows characteristics different from the ortho-hydroxylation enzymes. Significance: This is the first report on the oxygenation mechanism of a para-hydroxylating flavoenzyme. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

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URI

https://repository.li.mahidol.ac.th/handle/123456789/31142

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Scopus 2011-2015