Publication: The reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycle
| dc.contributor.author | Jeerus Sucharitakul | en_US |
| dc.contributor.author | Chanakan Tongsook | en_US |
| dc.contributor.author | Danaya Pakotiprapha | en_US |
| dc.contributor.author | Willem J.H. Van Berkel | en_US |
| dc.contributor.author | Pimchai Chaiyen | en_US |
| dc.contributor.other | Chulalongkorn University | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | Wageningen University and Research Centre | en_US |
| dc.date.accessioned | 2018-10-19T04:33:41Z | |
| dc.date.available | 2018-10-19T04:33:41Z | |
| dc.date.issued | 2013-12-06 | en_US |
| dc.description.abstract | Background: 3-Hydroxybenzoate 6-hydroxylase (3HB6H) is a flavoprotein hydroxylase catalyzing the para-hydroxylation of 3-hydroxybenzoate. Results: The oxidative half-reaction was studied using transient kinetics. The enzyme reaction mechanism was elucidated. Conclusion: The product release and the hydroxylation limit the turnovers and the enzyme shows characteristics different from the ortho-hydroxylation enzymes. Significance: This is the first report on the oxygenation mechanism of a para-hydroxylating flavoenzyme. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. | en_US |
| dc.identifier.citation | Journal of Biological Chemistry. Vol.288, No.49 (2013), 35222-35236 | en_US |
| dc.identifier.doi | 10.1074/jbc.M113.515205 | en_US |
| dc.identifier.issn | 1083351X | en_US |
| dc.identifier.issn | 00219258 | en_US |
| dc.identifier.other | 2-s2.0-84894312047 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/123456789/31142 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84894312047&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.title | The reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycle | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84894312047&origin=inward | en_US |