Publication:
Detection of herpes simplex virus type 2 Bgl II N fragment in paraffin-embedded cervical tissue sections using nested polymerase chain reaction

Issued Date

1994-01-01

Resource Type

Article

ISSN

08908508

DOI

10.1006/mcpr.1994.1063

Other identifier(s)

2-s2.0-0028566611

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Molecular and Cellular Probes. Vol.8, No.6 (1994), 441-447

Suggested Citation

Viraphong Lulitanond, Wasun Chantratita, Kamthorn Thammaprasert, Kanchana Nimmanahaeminda, Pornchai Matangkasombut, Chalobon Yoosook Detection of herpes simplex virus type 2 Bgl II N fragment in paraffin-embedded cervical tissue sections using nested polymerase chain reaction. Molecular and Cellular Probes. Vol.8, No.6 (1994), 441-447. doi:10.1006/mcpr.1994.1063 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9534

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Title

Detection of herpes simplex virus type 2 Bgl II N fragment in paraffin-embedded cervical tissue sections using nested polymerase chain reaction

Author(s)

Viraphong Lulitanond
 Wasun Chantratita
 Kamthorn Thammaprasert
 Kanchana Nimmanahaeminda
 Pornchai Matangkasombut
 Chalobon Yoosook

Other Contributor(s)

Mahidol University
 Faculty of Assoc. Medical Sciences
 Chiang Mai University

Abstract

To study the presence of transforming sequence Bgl II N of HSV-2 in cervical cancer tissues, we developed the nested polymerase chain reaction (PCR) for detecting such a sequence in paraffinembedded cervical tissue sections. Samples derived from 46 patients with premalignant and malignant lesions were tested. The sequence was found in 20-25% of total cases tested but not observed in any of the normal healthy controls. This study also indicates that for the detection of HSV-2 Bgl II N sequence in cervical tissue, the nested PCR may be more reliable than the in situ hybridization method. © 1994 Academic Press.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/9534

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Scopus 1991-2000