Publication: Fly-ash as a low-cost material for isolation of phosphoproteins
Issued Date
2018-12-01
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ISSN
18791298
00456535
00456535
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2-s2.0-85053765744
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Mahidol University
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SCOPUS
Bibliographic Citation
Chemosphere. Vol.213, (2018), 124-132
Suggested Citation
Pongsakorn Thawornpan, Supinya Thanapongpichat, Aung Win Tun, Amornrat Phongdara, Luitzen de Jong, Hansuk Buncherd Fly-ash as a low-cost material for isolation of phosphoproteins. Chemosphere. Vol.213, (2018), 124-132. doi:10.1016/j.chemosphere.2018.08.150 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45462
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Title
Fly-ash as a low-cost material for isolation of phosphoproteins
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Abstract
© 2018 Elsevier Ltd Metal oxide affinity chromatography (MOAC) is one of the most commonly used techniques for selective isolation phosphoproteins and phosphopeptides. This technique is capable of capturing the phosphorylated biomolecules through the affinity of the phosphoryl group for metal oxides/hydroxides. Fly-ash (FA), a by-product of coal-combustion power plants, is primarily composed of oxides of silicon and metals, among which iron and titanium. A number of studies have demonstrated the potential of these metal oxides for phosphoprotein and phosphopeptide enrichment. FA is annually produced over hundred million tons worldwide and generally considered as hazardous waste. It is thus of great importance to enhance its utilization. Here we present the first demonstration of the utility of FA as a low-cost MOAC material for the enrichment of phosphoproteins. With an FA-microcolumn, phosphoproteins can be successfully sequestered from other proteins. FA-microcolumns are shown to be simple, cheap and selective devices for phosphoprotein enrichment from a small volume of mixtures.