Publication:
Fly-ash as a low-cost material for isolation of phosphoproteins

dc.contributor.authorPongsakorn Thawornpanen_US
dc.contributor.authorSupinya Thanapongpichaten_US
dc.contributor.authorAung Win Tunen_US
dc.contributor.authorAmornrat Phongdaraen_US
dc.contributor.authorLuitzen de Jongen_US
dc.contributor.authorHansuk Buncherden_US
dc.contributor.otherSwammerdam Institute for Life Sciencesen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherPrince of Songkla Universityen_US
dc.date.accessioned2019-08-23T10:47:22Z
dc.date.available2019-08-23T10:47:22Z
dc.date.issued2018-12-01en_US
dc.description.abstract© 2018 Elsevier Ltd Metal oxide affinity chromatography (MOAC) is one of the most commonly used techniques for selective isolation phosphoproteins and phosphopeptides. This technique is capable of capturing the phosphorylated biomolecules through the affinity of the phosphoryl group for metal oxides/hydroxides. Fly-ash (FA), a by-product of coal-combustion power plants, is primarily composed of oxides of silicon and metals, among which iron and titanium. A number of studies have demonstrated the potential of these metal oxides for phosphoprotein and phosphopeptide enrichment. FA is annually produced over hundred million tons worldwide and generally considered as hazardous waste. It is thus of great importance to enhance its utilization. Here we present the first demonstration of the utility of FA as a low-cost MOAC material for the enrichment of phosphoproteins. With an FA-microcolumn, phosphoproteins can be successfully sequestered from other proteins. FA-microcolumns are shown to be simple, cheap and selective devices for phosphoprotein enrichment from a small volume of mixtures.en_US
dc.identifier.citationChemosphere. Vol.213, (2018), 124-132en_US
dc.identifier.doi10.1016/j.chemosphere.2018.08.150en_US
dc.identifier.issn18791298en_US
dc.identifier.issn00456535en_US
dc.identifier.other2-s2.0-85053765744en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/45462
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053765744&origin=inwarden_US
dc.subjectChemistryen_US
dc.subjectEnvironmental Scienceen_US
dc.titleFly-ash as a low-cost material for isolation of phosphoproteinsen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053765744&origin=inwarden_US

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