Publication: Simple and sensitive high-performance liquid chromatographic method for the determination of mycophenolic acid in plasma
Issued Date
2000-01-28
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ISSN
13872273
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2-s2.0-0033978697
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Chromatography B: Biomedical Sciences and Applications. Vol.738, No.1 (2000), 169-173
Suggested Citation
K. Na-Bangchang, O. Supasyndh, T. Supaporn, V. Banmairuroi, J. Karbwang Simple and sensitive high-performance liquid chromatographic method for the determination of mycophenolic acid in plasma. Journal of Chromatography B: Biomedical Sciences and Applications. Vol.738, No.1 (2000), 169-173. doi:10.1016/S0378-4347(99)00487-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25917
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Title
Simple and sensitive high-performance liquid chromatographic method for the determination of mycophenolic acid in plasma
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Abstract
A rapid, selective, sensitive, and reproducible reversed-phase HPLC procedure for the quantitative determination of mycophenolic acid (MPA) - an active plasma metabolite of the immunosuppressant mycophenolate mofetil (MMF) in plasma is described. The procedure involves one-step extraction of MPA and the internal standard, standard [RS-60461-000: (E)-6-[1,3-dihydro-4-(4-carboxy-butoxy)-6-methoxy-7-methyl-3-oxo-5-isobenzo-furanyl-4-methyl-4-hexenoic acid] with dichloromethane-dichloroethane (1:1, v/v) at acidic pH. Chromatographic separation consisted of the mobile phase [acetonitrile-0.05% phosphate buffer, pH 3.4 (45:55, v/v)] running through the column (Techopak-10 C18) at flow-rate of 0.8 ml/min. Detection was at UV wavelength of 254 nm. The mean recoveries of MPA and the internal standard at concentrations of 0.1 and 20 μg/ml were 89-98%, and 90-96%, respectively. The within-day coefficients of variation for MPA were 0.3-7.8% and the day-to-day coefficients of variation were 1.1-2.0%. The minimum detectable concentrations for both MPA and the internal standard in plasma were 0.005 μg/ml. The method was found to be suitable for use in clinical pharmacokinetic study. Copyright (C) 2000 Elsevier Science B.V.