Publication: Purification and characterization of anti-HIV-1 protein from Canna indica L. leaves
3
Issued Date
2012-11-05
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-84868144577
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.43, No.5 (2012), 1153-1159
Suggested Citation
Apanchanid Thepouyporn, Chalobon Yoosook, Wongsatit Chuakul, Krit Thirapanmethee, Chanita Napaswad, Chanpen Wiwat Purification and characterization of anti-HIV-1 protein from Canna indica L. leaves. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.43, No.5 (2012), 1153-1159. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14527
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Purification and characterization of anti-HIV-1 protein from Canna indica L. leaves
Other Contributor(s)
Abstract
A novel 10 kDa protein with anti-HIV-1 reverse transcriptase (RT) inhibitory activity was isolated from leaves of Canna indica L. using a combination of native-PAGE and ammonium sulfate precipitation. HIV-1 and RT inhibitory activity was measured using a syncytium forming ΔTat/Rev MC99 virus in Tat/Rev transfected 1A2 cell line and ELISA technique, respectively. Edman N-terminal and internal amino acid sequence (using LC-MS-MS) determination revealed the 10 kDa Canna indica L. leaf protein as a putative plastocyanin. This is the first report of a plant plastocyanin with HIV-1 RT inhibitory property.