Publication: Structural evidence of glycoprotein assembly in cellular membrane compartments prior to alphavirus budding
Issued Date
2010-01-01
Resource Type
ISSN
10985514
0022538X
0022538X
Other identifier(s)
2-s2.0-77957957373
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virology. Vol.84, No.21 (2010), 11145-11151
Suggested Citation
Pan Soonsawad, Li Xing, Emerson Milla, Juan M. Espinoza, Masaaki Kawano, Michael Marko, Chyongere Hsieh, Hiromitsu Furukawa, Masahiro Kawasaki, Wattana Weerachatyanukul, Ranjana Srivastava, Susan W. Barnett, Indresh K. Srivastava, R. Holland Cheng Structural evidence of glycoprotein assembly in cellular membrane compartments prior to alphavirus budding. Journal of Virology. Vol.84, No.21 (2010), 11145-11151. doi:10.1128/JVI.00036-10 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28557
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Title
Structural evidence of glycoprotein assembly in cellular membrane compartments prior to alphavirus budding
Abstract
Membrane glycoproteins of alphavirus play a critical role in the assembly and budding of progeny virions. However, knowledge regarding transport of viral glycoproteins to the plasma membrane is obscure. In this study, we investigated the role of cytopathic vacuole type II (CPV-II) through in situ electron tomography of alphavirus-infected cells. The results revealed that CPV-II contains viral glycoproteins arranged in helical tubular arrays resembling the basic organization of glycoprotein trimers on the envelope of the mature virions. The location of CPV-II adjacent to the site of viral budding suggests a model for the transport of structural components to the site of budding. Thus, the structural characteristics of CPV-II can be used in evaluating the design of a packaging cell line for replicon production. Copyright © 2010, American Society for Microbiology. All Rights Reserved.