Publication: BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei
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Issued Date
2010-12-09
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ISSN
19326203
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2-s2.0-78649735350
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS ONE. Vol.5, No.11 (2010)
Suggested Citation
Jolene R. Bowers, David M. Engelthaler, Jennifer L. Ginther, Talima Pearson, Sharon J. Peacock, Apichai Tuanyok, David M. Wagner, Bart J. Currie, Paul S. Keim BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei. PLoS ONE. Vol.5, No.11 (2010). doi:10.1371/journal.pone.0015413 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28419
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Title
BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei
Abstract
A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei. © 2010 Bowers et al.