Publication: BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei
| dc.contributor.author | Jolene R. Bowers | en_US |
| dc.contributor.author | David M. Engelthaler | en_US |
| dc.contributor.author | Jennifer L. Ginther | en_US |
| dc.contributor.author | Talima Pearson | en_US |
| dc.contributor.author | Sharon J. Peacock | en_US |
| dc.contributor.author | Apichai Tuanyok | en_US |
| dc.contributor.author | David M. Wagner | en_US |
| dc.contributor.author | Bart J. Currie | en_US |
| dc.contributor.author | Paul S. Keim | en_US |
| dc.contributor.other | Translational Genomics Research Institute | en_US |
| dc.contributor.other | Northern Arizona University | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | Menzies School of Health Research | en_US |
| dc.date.accessioned | 2018-09-24T08:37:00Z | |
| dc.date.available | 2018-09-24T08:37:00Z | |
| dc.date.issued | 2010-12-09 | en_US |
| dc.description.abstract | A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei. © 2010 Bowers et al. | en_US |
| dc.identifier.citation | PLoS ONE. Vol.5, No.11 (2010) | en_US |
| dc.identifier.doi | 10.1371/journal.pone.0015413 | en_US |
| dc.identifier.issn | 19326203 | en_US |
| dc.identifier.other | 2-s2.0-78649735350 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/28419 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649735350&origin=inward | en_US |
| dc.subject | Agricultural and Biological Sciences | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.subject | Medicine | en_US |
| dc.title | BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649735350&origin=inward | en_US |