Publication: Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei
Issued Date
1999-11-01
Resource Type
ISSN
00951137
Other identifier(s)
2-s2.0-0032867180
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Microbiology. Vol.37, No.11 (1999), 3742-3745
Suggested Citation
Sunee Korbsrisate, Nuttiga Suwanasai, Amornrut Leelaporn, Takayuki Ezaki, Yoshiaki Kawamura, Suttipant Sarasombath Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei. Journal of Clinical Microbiology. Vol.37, No.11 (1999), 3742-3745. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25569
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Title
Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei
Abstract
We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic PLC from Pseudomonas aeruginosa. The expressed PC-PLC was heat stable, nonhemolytic for sheep erythrocytes, and active between pH 2 and 8. Western blot analysis with sera from melioidosis patients indicated that they produced immunoglobulin M antibodies against this PC-PLC protein.