Publication: Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei
| dc.contributor.author | Sunee Korbsrisate | en_US |
| dc.contributor.author | Nuttiga Suwanasai | en_US |
| dc.contributor.author | Amornrut Leelaporn | en_US |
| dc.contributor.author | Takayuki Ezaki | en_US |
| dc.contributor.author | Yoshiaki Kawamura | en_US |
| dc.contributor.author | Suttipant Sarasombath | en_US |
| dc.contributor.other | Faculty of Medicine, Siriraj Hospital, Mahidol University | en_US |
| dc.contributor.other | Gifu University School of Medicine | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.date.accessioned | 2018-09-07T08:54:51Z | |
| dc.date.available | 2018-09-07T08:54:51Z | |
| dc.date.issued | 1999-11-01 | en_US |
| dc.description.abstract | We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic PLC from Pseudomonas aeruginosa. The expressed PC-PLC was heat stable, nonhemolytic for sheep erythrocytes, and active between pH 2 and 8. Western blot analysis with sera from melioidosis patients indicated that they produced immunoglobulin M antibodies against this PC-PLC protein. | en_US |
| dc.identifier.citation | Journal of Clinical Microbiology. Vol.37, No.11 (1999), 3742-3745 | en_US |
| dc.identifier.issn | 00951137 | en_US |
| dc.identifier.other | 2-s2.0-0032867180 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/25569 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032867180&origin=inward | en_US |
| dc.subject | Medicine | en_US |
| dc.title | Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032867180&origin=inward | en_US |