Publication: Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus
Issued Date
2013-05-01
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ISSN
00029637
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2-s2.0-84877115468
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.88, No.5 (2013), 932-936
Suggested Citation
Rattanaphone Phetsouvanh, Thaksinaporn Thojaikong, Phonlavanh Phoumin, Bountoy Sibounheuang, Koukeo Phommasone, Vilada Chansamouth, Sue J. Lee, Paul N. Newton, Stuart D. Blacksell Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus. American Journal of Tropical Medicine and Hygiene. Vol.88, No.5 (2013), 932-936. doi:10.4269/ajtmh.12-0325 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31924
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Title
Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus
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Abstract
Inter- and intra-observer variation was examined among six microscopists who read 50 scrub typhus (ST) and murine typhus (MT) indirect immunofluorescence assay (IFA) immunoglobulinM(IgM) slides. Inter-observer agreement was moderate (κ = 0.45) for MT and fair (κ = 0.32) for ST, and was significantly correlated with experience (P = 0.03 and P = 0.004, respectively); k-scores for intra-observer agreement between morning and afternoon readings (range = 0.35-0.86) were not correlated between years of experience for ST and MT IFAs (Spearman's ρ = 0.31, P = 0.54 and P = 0.14, respectively; P = 0.78). Storage at 4°C for 2 days showed a change from positive to negative in 20-32% of slides. Although the titers did not dramatically change after 14 days of storage, the final interpretation (positive to negative) did change in 36-50% of samples, and it, therefore, recommended that slides should be read as soon as possible after processing. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.