Publication: A simple and rapid liquid chromatographic assay for evaluation of potentially counterfeit Tamiflu®
Issued Date
2006-11-01
Resource Type
ISSN
07317085
Other identifier(s)
2-s2.0-33748533177
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.42, No.4 (2006), 430-433
Suggested Citation
N. Lindegårdh, T. T. Hien, J. Farrar, P. Singhasivanon, N. J. White, N. P J Day A simple and rapid liquid chromatographic assay for evaluation of potentially counterfeit Tamiflu®. Journal of Pharmaceutical and Biomedical Analysis. Vol.42, No.4 (2006), 430-433. doi:10.1016/j.jpba.2006.04.028 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/23144
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Title
A simple and rapid liquid chromatographic assay for evaluation of potentially counterfeit Tamiflu®
Abstract
A simple and rapid liquid chromatographic assay for the evaluation of potentially counterfeit oseltamivir (Tamiflu®) has been developed and assessed. The assay uses approximately 1 mg Tamiflu®powder when used for authentication and content estimate. The procedure was validated using 50 replicates analysed during five independent series with a total R.S.D. of 11.2%. The assay can also be used to monitor the exact content of oseltamivir in Tamiflu®capsules. One Tamiflu®capsule was transferred to a 250 mL volumetric flask and 150 mL water was added. The flask was placed in an ultrasonic bath at 40 °C for 20 min to dissolve the capsule. The solution was allowed to cool to room temperature before the flask was filled up to the mark (250 mL). A small aliquot was centrifuged and then directly injected into the LC-system for quantification. Oseltamivir was analysed by liquid chromatography with UV detection on a Hypersil Gold column (150 mm × 4.6 mm) using a mobile phase containing methanol-phosphate buffer (pH 2.5; 0.1 M) (50:50, v/v) at a flow rate of 1.0 mL/min. The assay was implemented for the analysis of Tamiflu®purchased over the Internet and at local pharmacies in Thailand and Vietnam. © 2006 Elsevier B.V. All rights reserved.