Publication:
Identification of Dengue virus-infected cells in paraffin-embedded tissue using in situ polymerase chain reaction and DNA hybridization

Issued Date

1994-01-01

Resource Type

Article

ISSN

01660934

DOI

10.1016/0166-0934(94)90083-3

Other identifier(s)

2-s2.0-0028103955

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Virological Methods. Vol.48, No.1 (1994), 1-8

Suggested Citation

Helen L. Lucia, Daoroong Kangwanpong Identification of Dengue virus-infected cells in paraffin-embedded tissue using in situ polymerase chain reaction and DNA hybridization. Journal of Virological Methods. Vol.48, No.1 (1994), 1-8. doi:10.1016/0166-0934(94)90083-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9583

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Title

Identification of Dengue virus-infected cells in paraffin-embedded tissue using in situ polymerase chain reaction and DNA hybridization

Author(s)

Helen L. Lucia
 Daoroong Kangwanpong

Other Contributor(s)

University of Mississippi Medical Center
 Mahidol University

Abstract

Using tissue from suckling mice infected with Dengue virus, the following improved method for detecting Dengue viral RNA in tissue sections was devised. Reverse transcription of the viral RNA to DNA was followed by the in situ polymerase chain reaction to amplify the viral nucleic acid. This was followed by DNA hybridization with an alkaline phosphatase-labelled probe. The enzyme was then reacted with Fast red and counterstained with hematoxylin. Viral nucleic acid was readily demonstrated within glial cells and macrophages in brains of animals infected with two different serotypes of Dengue. © 1994.

Keyword(s)

Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/9583

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Scopus 1991-2000