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Identification of Dengue virus-infected cells in paraffin-embedded tissue using in situ polymerase chain reaction and DNA hybridization

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dc.contributor.authorHelen L. Luciaen_US
dc.contributor.authorDaoroong Kangwanpongen_US
dc.contributor.otherUniversity of Mississippi Medical Centeren_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-02-27T04:26:36Z
dc.date.available2018-02-27T04:26:36Z
dc.date.issued1994-01-01en_US
dc.description.abstractUsing tissue from suckling mice infected with Dengue virus, the following improved method for detecting Dengue viral RNA in tissue sections was devised. Reverse transcription of the viral RNA to DNA was followed by the in situ polymerase chain reaction to amplify the viral nucleic acid. This was followed by DNA hybridization with an alkaline phosphatase-labelled probe. The enzyme was then reacted with Fast red and counterstained with hematoxylin. Viral nucleic acid was readily demonstrated within glial cells and macrophages in brains of animals infected with two different serotypes of Dengue. © 1994.en_US
dc.identifier.citationJournal of Virological Methods. Vol.48, No.1 (1994), 1-8en_US
dc.identifier.doi10.1016/0166-0934(94)90083-3en_US
dc.identifier.issn01660934en_US
dc.identifier.other2-s2.0-0028103955en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/9583
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0028103955&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.titleIdentification of Dengue virus-infected cells in paraffin-embedded tissue using in situ polymerase chain reaction and DNA hybridizationen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0028103955&origin=inwarden_US

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