Panadda Boonserm, David J. Ellar, Jade Li Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp. israelensis. Acta Crystallographica - Section D Biological Crystallography. Vol.59, No.3 (2003), 591-594. doi:10.1107/S0907444903001513 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20758
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Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp. israelensis
The Cry4B δ-endotoxin from Bacillus thuringiensis subsp. israelensis is specifically toxic to mosquito larvae. For a better understanding of the mechanism of toxicity, chymotrypsin-activated Cry4B toxin (68 kDa) has been purified and crystallized in sodium bromide at neutral pH. The well formed crystals belong to the rhombohedral space group R32, with unit-cell parameters a = b = 185.82, c = 187.93 Å, and diffracted X-rays to 1.75 Å resolution. The asymmetric unit contains one toxin molecule and 74% solvent content, as shown by molecular replacement from a composite model of the homologous Cry3A and Cry1Aa. The purified protein and crystals both possessed mosquitocidal activity.
