Publication:
Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp. israelensis

1

Issued Date

2003-03-01

Resource Type

Article

ISSN

09074449

DOI

10.1107/S0907444903001513

Other identifier(s)

2-s2.0-0037348708

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Acta Crystallographica - Section D Biological Crystallography. Vol.59, No.3 (2003), 591-594

Suggested Citation

Panadda Boonserm, David J. Ellar, Jade Li Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp. israelensis. Acta Crystallographica - Section D Biological Crystallography. Vol.59, No.3 (2003), 591-594. doi:10.1107/S0907444903001513 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20758

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Title

Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp. israelensis

Author(s)

Panadda Boonserm
 David J. Ellar
 Jade Li

Other Contributor(s)

University of Cambridge
 The Medical Research Council Laboratory of Molecular Biology
 Mahidol University

Abstract

The Cry4B δ-endotoxin from Bacillus thuringiensis subsp. israelensis is specifically toxic to mosquito larvae. For a better understanding of the mechanism of toxicity, chymotrypsin-activated Cry4B toxin (68 kDa) has been purified and crystallized in sodium bromide at neutral pH. The well formed crystals belong to the rhombohedral space group R32, with unit-cell parameters a = b = 185.82, c = 187.93 Å, and diffracted X-rays to 1.75 Å resolution. The asymmetric unit contains one toxin molecule and 74% solvent content, as shown by molecular replacement from a composite model of the homologous Cry3A and Cry1Aa. The purified protein and crystals both possessed mosquitocidal activity.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/20758

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Scopus 2001-2005