Publication: Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp. israelensis
| dc.contributor.author | Panadda Boonserm | en_US |
| dc.contributor.author | David J. Ellar | en_US |
| dc.contributor.author | Jade Li | en_US |
| dc.contributor.other | University of Cambridge | en_US |
| dc.contributor.other | The Medical Research Council Laboratory of Molecular Biology | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.date.accessioned | 2018-07-24T03:20:43Z | |
| dc.date.available | 2018-07-24T03:20:43Z | |
| dc.date.issued | 2003-03-01 | en_US |
| dc.description.abstract | The Cry4B δ-endotoxin from Bacillus thuringiensis subsp. israelensis is specifically toxic to mosquito larvae. For a better understanding of the mechanism of toxicity, chymotrypsin-activated Cry4B toxin (68 kDa) has been purified and crystallized in sodium bromide at neutral pH. The well formed crystals belong to the rhombohedral space group R32, with unit-cell parameters a = b = 185.82, c = 187.93 Å, and diffracted X-rays to 1.75 Å resolution. The asymmetric unit contains one toxin molecule and 74% solvent content, as shown by molecular replacement from a composite model of the homologous Cry3A and Cry1Aa. The purified protein and crystals both possessed mosquitocidal activity. | en_US |
| dc.identifier.citation | Acta Crystallographica - Section D Biological Crystallography. Vol.59, No.3 (2003), 591-594 | en_US |
| dc.identifier.doi | 10.1107/S0907444903001513 | en_US |
| dc.identifier.issn | 09074449 | en_US |
| dc.identifier.other | 2-s2.0-0037348708 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/20758 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0037348708&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.title | Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp. israelensis | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0037348708&origin=inward | en_US |