Publication: Establishment of a rabbit induced pluripotent stem cell (RbiPSC) line using lentiviral delivery of human pluripotency factors
Issued Date
2017-05-01
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ISSN
18767753
18735061
18735061
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2-s2.0-85015993999
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cell Research. Vol.21, (2017), 16-18
Suggested Citation
Zsuzsanna Táncos, Csilla Nemes, Eszter Varga, István Bock, Sasitorn Rungarunlert, Theerawat Tharasanit, Mongkol Techakumphu, Julianna Kobolák, András Dinnyés Establishment of a rabbit induced pluripotent stem cell (RbiPSC) line using lentiviral delivery of human pluripotency factors. Stem Cell Research. Vol.21, (2017), 16-18. doi:10.1016/j.scr.2017.03.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41885
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Title
Establishment of a rabbit induced pluripotent stem cell (RbiPSC) line using lentiviral delivery of human pluripotency factors
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Abstract
© 2017 The Authors Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. Furthermore, the spontaneous differentiation potential of the iPSC was also tested in vivo by teratoma assay. The iPSC line showed normal karyotype. The advantages of using RbiPSC are the easy access to primary material and the possibility to study the efficacy and safety of the iPSC-based therapies on a non-rodent animal model.