Structural analysis of jack fruit lectin
2
Issued Date
2024
Copyright Date
1989
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xi, 157 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 1989
Suggested Citation
Wanwisa Srisukh Structural analysis of jack fruit lectin. Thesis (M.Sc. (Biochemistry))--Mahidol University, 1989. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/100263
Title
Structural analysis of jack fruit lectin
Alternative Title(s)
โครงสร้างของเลคตินจากเมล็ดขนุน
Author(s)
Abstract
The structure of Jack fruit lectin (JFL) from the seeds of Jack fruit (Artocarpus heterophyllus) was studied using various techniques: proteolytic cleavage, peptide mapping, chemical modification and subunit dissociation by sodium dodecyl sulphate. Hemagglutinating activity of JFL was most sensitive to pronase digestion. It was also sensitive to subtilisin, papain, and was less sensitive to trypsin, cyhmotrypsin, thermolysin. By gel filtration, the lectin activity was found to reside with intact JFL but not any digested product. JFL activity was completely lost after chemical modifications of 5 lysine and 19 tryptophan residues. Inactivity was observed after modification of carboxyl groups, tyrosine or histidine. These residues were probably required for maintaining the active conformation. Subunits of JFL was not linked by disulfide bonding but could be dissociated by 0.01% (w/v) sodium dodecyl sulfate which also caused the loss of its sugar-binding capacity. JFL and Jacalin from Artocarpus inteqrifolia were shown to have similar structures by peptide mapping and similar subunit compositions by electrophoresis in sodium dodecyl sulfate.
Description
Biochemistry (Mahidol University 1989)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University