Characterization of ribosomal DNA clones from rice
1
Issued Date
2024
Copyright Date
1993
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xi, 142 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 1993
Suggested Citation
Pongsopee Attasart Characterization of ribosomal DNA clones from rice. Thesis (M.Sc. (Biochemistry))--Mahidol University, 1993. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/101022
Title
Characterization of ribosomal DNA clones from rice
Alternative Title(s)
การศึกษาคุณลักษณะของโคลนที่มีไรโบโซมัลดีเอ็นเอจากข้าว
Author(s)
Advisor(s)
Abstract
A 3,699 base pairs long nucleotide sequence of an indica rice (KDML105) DNA clone, called GX3.6, was determined. Five additional DNA clones, designated as C13, C14, C32, C35 and C76, were also isolated from a KDML105 rice cDNA library and DNA sequences determined. The 5 new clones showed approximately 5777 % sequence matching with that of GX3.6. Identity searching from DNA data banks showed that the GX3.6 and the new DNA clones had high sequence identities with large subunit rRNA genes of plant organelles. Nucleotide sequence comparison between GX3.6 and known rRNA genes revealed the presence of three conserved regions in GX3.6 which showed 50-60 % sequence identities with 23S rRNA gene (2808 bp in length), 4.5S rRNA gene (95 bp in length) and 5S rRNA gene (118 bp in length) of plant chloroplasts. The structural and arrangement of 3 putative genes in GX3.6 were similar to those of plant chloroplast rRNA genes. However, a copy of GX3.6 DNA could not be detected in rice chloroplast DNA and nuclear DNA based on Southern hybridization of total DNAs from rice leaves. Many data available indicated the clone GX3.6 was likely from rice mitochondrial origin. However, the DNA clones C32, C35 and C76 were identified as parts of indica rice chloroplast 23S rRNA gene.
Description
Biochemistry (Mahidol University 1993)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University