Temporomandibular Joint Pain Measurement by Bite Force and Von Frey Filament Assays in Mice
1
Issued Date
2024-09-13
Resource Type
eISSN
1940087X
DOI
Scopus ID
2-s2.0-85205335987
Pubmed ID
39345152
Journal Title
Journal of visualized experiments : JoVE
Issue
211
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of visualized experiments : JoVE No.211 (2024)
Suggested Citation
Jariyasakulroj S., Chang Q., Ko P.F., Shu Y., Lin Z., Chen J., Chen J.F. Temporomandibular Joint Pain Measurement by Bite Force and Von Frey Filament Assays in Mice. Journal of visualized experiments : JoVE No.211 (2024). doi:10.3791/67203 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/101514
Title
Temporomandibular Joint Pain Measurement by Bite Force and Von Frey Filament Assays in Mice
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Abstract
Temporomandibular joint (TMJ) pain and osteoarthritis (OA) are common and debilitating disorders that impair patients' quality of life. The mechanisms driving diseases-related pain are poorly understood, and current treatments fail to provide effective and long-term therapeutic effects. Additionally, pain assessment in research, particularly orofacial pain, poses several challenges that complicate studies in both clinical and basic science settings. Therefore, we have established an inflammatory TMJ pain mouse model via intra-articular injection of CFA (Complete Freund's Adjuvant) and evaluated pain behaviors by bite force measurement and the von Frey filament test. Mice with CFA injection exhibited orofacial pain behaviors compared to PBS injection, including reduced bite force and head withdrawal threshold in the von Frey filament test. These methods are relatively easy to execute to have reproducible results and can be potentially extended to pain studies for other disease models related to TMJ disorders. Together, bite force, and the von Frey filament tests are reliable in measuring orofacial pain, as demonstrated in CFA injection-induced painful TMJOA mouse models.