Enhancement of dark fermentative hydrogen production using metal-modified biochar from sugarcane residues: Optimization, characterization, and metabolic analysis
Issued Date
2025-04-01
Resource Type
ISSN
03014797
eISSN
10958630
Scopus ID
2-s2.0-105000146721
Journal Title
Journal of Environmental Management
Volume
380
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Environmental Management Vol.380 (2025)
Suggested Citation
Wongfaed N., Sittijunda S., O-Thong S., Kongjan P., Jariyaboon R., Plangklang P., Reungsang A. Enhancement of dark fermentative hydrogen production using metal-modified biochar from sugarcane residues: Optimization, characterization, and metabolic analysis. Journal of Environmental Management Vol.380 (2025). doi:10.1016/j.jenvman.2025.125047 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/108507
Title
Enhancement of dark fermentative hydrogen production using metal-modified biochar from sugarcane residues: Optimization, characterization, and metabolic analysis
Corresponding Author(s)
Other Contributor(s)
Abstract
This study investigated the enhancement of dark fermentative hydrogen production (HP) using metal-modified biochars derived from sugarcane bagasse (SB) and sugarcane leaves (SL). The biochars were modified with Fe and Ni, with optimal conditions identified through Box-Behnken Design as 6.09 g/L biochar: SB, 5.38 g/L biochar: SL + Fe, and 7.66 g/L biochar: SL + Ni. This optimization achieved a maximum hydrogen yield of 108.77 mL-hydrogen (H2)/g-glucose, a 58.77 % increase over the control. Metal modification enhanced biochar surface properties and selectively enriched H2-producing bacteria, particularly Clostridium sensu stricto 1 and Paraclostridium sp. Metabolic pathway analysis showed enhanced glucose catabolism and increased H2-producing enzyme abundance. The study demonstrates that sugarcane-derived biochar can effectively enhance bio-HP, though careful optimization of metal concentrations is crucial to avoid inhibitory effects.