Ganciclovir-Loaded Chitosan Nanoparticles and Their Activity against HSV-1 Inducing Herpetic Retinitis
7
Issued Date
2025-04-01
Resource Type
ISSN
25281410
eISSN
25278045
Scopus ID
2-s2.0-105006531920
Journal Title
Indonesian Journal of Science and Technology
Volume
10
Issue
1
Start Page
171
End Page
190
Rights Holder(s)
SCOPUS
Bibliographic Citation
Indonesian Journal of Science and Technology Vol.10 No.1 (2025) , 171-190
Suggested Citation
Haruna L.A., Purnama L.O.M.J., Fristiohady A., Masrinoul P., Komaikul J., Ketsuwan K., Asasutjarit R. Ganciclovir-Loaded Chitosan Nanoparticles and Their Activity against HSV-1 Inducing Herpetic Retinitis. Indonesian Journal of Science and Technology Vol.10 No.1 (2025) , 171-190. 190. doi:10.17509/ijost.v10i1.80998 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/110464
Title
Ganciclovir-Loaded Chitosan Nanoparticles and Their Activity against HSV-1 Inducing Herpetic Retinitis
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Abstract
Herpes simplex virus (HSV)-1-induced retinitis is one of ophthalmic viral infections. It is found in some immunocompromised and elderly patients and is a cause of complete blindness. Nowadays, an intravitreal (IVT) injection of ganciclovir (GCV) is applied to treat this disease. Unfortunately, frequent IVT injections induce adverse effects in the eye. GCV-loaded chitosan nanoparticles (GCV-CS-NPs) were developed to reduce the number of IVT injections. This study developed GCV-CS-NPs and investigated their activity against HSV-1. GCV-CS-NPs were prepared using an ionic gelation method with varying formulation compositions. The particle size, polydispersity index (PDI), zeta potential, pH, drug release, and activity against the HSV-1 were investigated. The results showed GCV-CS-NPs with particle sizes of around 161 to 294 nm, moderate PDI values, and zeta potentials of between +8.0 and +18.1 mV. These properties strongly depended on the formulation compositions. The release rate of GCV from the optimized GCV-CS-NPs (3C-0.5G-1T-4.5) in the simulated vitreous humor regarding Higuchi’s model was 4.89±0.48 µg/min1/2. The IC50 against the HSV-1 was 25.12±0.02 µg/mL. 3C-0.5G-1T-4.5 could be accepted as a promising delivery system of GCV to the vitreous humor via IVT injection and had a potential for being subjected to further in vivo studies.