Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology
Issued Date
2025-04-18
Resource Type
ISSN
00368075
eISSN
10959203
Scopus ID
2-s2.0-105008780258
Journal Title
Science
Volume
388
Issue
6744
Start Page
297
End Page
303
Rights Holder(s)
SCOPUS
Bibliographic Citation
Science Vol.388 No.6744 (2025) , 297-303
Suggested Citation
Sanguankiattichai N., Chandrasekar B., Sheng Y., Hardenbrook N., Tabak W.W.A., Drapal M., Kaschani F., Grünwald-Gruber C., Krahn D., Buscaill P., Yamamoto S., Kato A., Nash R., Fleet G., Strasser R., Fraser P.D., Kaiser M., Zhang P., Preston G.M., van der Hoorn R.A.L. Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology. Science Vol.388 No.6744 (2025) , 297-303. 303. doi:10.1126/science.adp2433 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/110981
Title
Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology
Author's Affiliation
Corresponding Author(s)
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Abstract
The extracellular space (apoplast) in plants is a key battleground during microbial infections. To avoid recognition, the bacterial model phytopathogen Pseudomonas syringae pv. tomato DC3000 produces glycosyrin. Glycosyrin inhibits the plant-secreted b-galactosidase BGAL1, which would otherwise initiate the release of immunogenic peptides from bacterial flagellin. Here, we report the structure, biosynthesis, and multifunctional roles of glycosyrin. High-resolution cryo–electron microscopy and chemical synthesis revealed that glycosyrin is an iminosugar with a five-membered pyrrolidine ring and a hydrated aldehyde that mimics monosaccharides. Glycosyrin biosynthesis was controlled by virulence regulators, and its production is common in bacteria and prevents flagellin recognition and alters the extracellular glycoproteome and metabolome of infected plants. These findings highlight a potentially wider role for glycobiology manipulation by plant pathogens across the plant kingdom.