Streptomyces sediminimaris sp. nov., a novel actinobacterium with anticancer potential isolated from mangrove sediments
Issued Date
2025-01-01
Resource Type
ISSN
14665026
eISSN
14665034
Scopus ID
2-s2.0-105008705780
Pubmed ID
40512541
Journal Title
International Journal of Systematic and Evolutionary Microbiology
Volume
75
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Systematic and Evolutionary Microbiology Vol.75 No.6 (2025)
Suggested Citation
Emthomya N., Chuangrattanawan C., Ngamcharungchit C., Kongsaya T., Kanchanasin P., Phongsopitanun W., Suriyachadkun C., Pidot S.J., Intra B. Streptomyces sediminimaris sp. nov., a novel actinobacterium with anticancer potential isolated from mangrove sediments. International Journal of Systematic and Evolutionary Microbiology Vol.75 No.6 (2025). doi:10.1099/ijsem.0.006811 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/110999
Title
Streptomyces sediminimaris sp. nov., a novel actinobacterium with anticancer potential isolated from mangrove sediments
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Corresponding Author(s)
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Abstract
Two marine actinomycete-like strains, MCC20<sup>T</sup> and MCC57, were isolated from Chanthaburi (Thailand) mangrove sediment. Their taxonomic classifications were established through a polyphasic approach. Despite differences in colony morphotypes, genetic and chemotaxonomic analyses confirmed them as the same species within the genus Streptomyces. Both strains contained ll-diaminopimelic acid in their cell wall, with glucose, mannose, ribose and rhamnose identified as whole-cell sugars. Their phospholipid profile comprises phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The predominant fatty acids were iso-C<inf>15:0</inf>, anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf> and anteiso-C<inf>17:0</inf> with MK-9(H<inf>8</inf>) as the primary menaquinone, while MK-9(H<inf>6</inf>) and MK-9(H<inf>4</inf>) were unique to strain MCC57. Both strains exhibited anticancer activity against colorectal (HCT116) and lung (A549) cancer cells, with strain MCC20<sup>T</sup> being more potent. Their 16S rRNA gene sequences showed 100% similarity, with 99.2% similarity to Streptomyces fumigatiscleroticus NBRC 12999<sup>T</sup>. Nevertheless, a phylogenomic tree placed them closer to Streptomyces spinosirectus CRSS-Y-16<sup>T</sup>, Streptomyces plumbidurans KC 17012<sup>T</sup> and Streptomyces spinosisporus 7R016<sup>T</sup>. Nearly 100% average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values highlighted the identity of strains MCC20<sup>T</sup> and MCC57, while ANI (89.4%) and dDDH (35.5%) values were well below the respective 95 and 70% thresholds for related species. This supported their novelty. Based on their genotypes and phenotypes, strains MCC20<sup>T</sup> (=NBRC 117131<sup>T</sup>=TBRC 19240<sup>T</sup>) and MCC57 (=NBRC 117132=TBRC 19241) are identified as phenotypic variants of a new species, Streptomyces sediminimaris sp. nov., with strain MCC20<sup>T</sup> designated as the type strain (~9.2 Mb genome, 72.0 mol% G+C content).